Metagenomics BioMaS workflow

The BioMaS (Bioinformatic analysis of Metagenomic AmpliconS) workflow has been designed for the taxonomic profiling of environmental microbial communities through target-oriented metagenomic surveys. It includes all the computational processing steps from raw Next Generation Sequencing (NGS) data input and cleaning to the final taxonomic identification and representation. In its current version, BioMaS allows the analysis of both bacterial and fungal environments starting directly from the output of 454 and illumina NGS platforms.

Who is it for?

All users who have microbial target-oriented metagenomic DNA sequence data generated by NGS platforms.

What is it for?

BioMaS is designed as a completely automated workflow, comprehensive of all the fundamental steps, from raw sequence data arrangement
to final taxonomic identification.

How does it works?

BioMaS includes the following consecutively and automatically running modules: 1.) raw data statistical and quality evaluation and arrangement for the next analysis steps; 2.) Mapping of sequence data on reference databases and resulting hits parsing; 3.) Taxonomical binning and assignment.

Expected results

BioMaS produces a graphical three and several pie-charts describing the taxonomic complexity of the investigated microbiota at different rank (from phylum to species). All the analysis results are embedded in a single html file (an example is shown in the figure below: a) the html report homepage, b) an example of the interactive pie-charts summarizing the taxonomic assignment, c) an example of the svg tree).

Links to workflow and user documentation


BioMaS: a modular pipeline for Bio-informatic analysis of Metagenomic AmpliconS. BMC Bioinformatics. Submitted.





19 February 2015

At the final review of the project by the EC, one of the reviewers said: “Incredible work done with a community that is not unified. Remarkable work. It opens for new development in a near future. Hope for success. Good project. Happy that you have been financed three plus years ago.”

Read all about the project and its results in the Project Final Report or read the Executive Summary only.